About

• AutoPure ADA can be used on various automated analyzers. General procedure is as described below.

ADA catalyzes deamination of adenosine to inosine which is then converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H,0) by xanthine oxidase (XOD).H,0, is further reacted with N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT) and 4-aminoantipyrine (4 AAP) in the presence of peroxidase (POD) to generate quinone dye.

Calibration frequency Re-calibration is recommended  as per the requirement of quality control procedures.

Others Details :

• The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
• The reagent and standard are ready-to-use and are stable till expiry, when stored at 2- 8°C. DO NOT FREEZE THE REAGENT.

Procedure :

Reaction direction…………………………. Increasing
Wavelength …………….. ….. 546 nm.
Flow Cell temperature …………………… 37°C
Zero setting with …… Distilled water
Delay time.. . ..300 seconds
No. of readings…………4
Interval………………… 60 seconds
Sample volume. ………….. 10 ul
Reagent 1 (R1) volume…………. 360 ul
Reagent 2 (R2) volume…… 180 ul
Factor. ……….. 1743
Linearity … …………..200 U/L