• AutoPure Alkaine Phosphatase is a reagent kit for direct quantitative determination of alkaline phosphatase in human serum and plasma on automated clinical chemistry analyzers.
• AutoPure Alkaline Phosphatase is a single reagent system.
• AutoPure Alkaline Phosphatase has a single step reconstitution. It involves reconstitution of substrate tablets with the diluent.
• With AutoPure Alkaline Phosphatase, the assay is linear upto 2000 IU/I (33.4ukat/I )

Shelf Life: Stable till expiry date indicated on Label

Principles :

Alkaline Phosphatase cleaves p-nitrophenyl phosphate pN
in the presence of magnesium ions, to form p nitrophenol and
phosphate.

The intensity of the yellow color famed is directly proportional to the alkaline Solution.

Procedure :

Reaction type ………… ………. End-Point
Reaction time …. 10 mins. at 37°C/20 mins. at R.T. (25-30°C)
Wavelength ……….. ………… 510 nm. (500 – 530 nm.)
Zero setting with ……….. ………… Reagent Blank
Blank absorbance limit … ……… < 0.300 Abs.
Sample volume …………………………… 0.01 ml (10 jul)
Reagent volume ……….. …………. 1.0 ml
Standard concentration .. …………… 200 mg%
Linearity ……………. ….. 800 mg/dl

Quality Controls :

Each batch of AutoPure Alkaline Phosphatase is assayed with at least six quality Conto sera PrecnomPrecpath Barad* Bio Rad l Accutrol Normal and AcCutrol Abnomal Prioritize To ensure adequate qua control tS Recommended that he laboratory should use a noma and abdomen pmmercarelerence cono Seru. it should be realized that the use of quality control matena checks both the reagent and instrument funchans igihe if the control values fall outside the specified limits, each the below criteria should
De cross-checked and corrected Proper instrument function- wavelength selling Light source and temperature control.