• AutoPure Amylase is reagent kit for direct quantitative determination of Amylase in human security and plagina on automated clinical chemistry analyzers.
• AutoPure Amylase is a ready-to-use, single liquid reagent system
• With AutoPure Amylase the assay is linear upto 2000 IU (33.4 phat).

Principles :

Amylase involves use of a chromogenic substrate Gal-G,-d-CNP 2- chloro-4-nitrophenyl linked with galactosyl maltoside). The Girec action of d-Amylase with this substrate results n ne release of more than 90% of 2-chloro-4-nitrophenol.

The intensity of the 2- chloro 4- nitrophenol formed is directly proportional to the -Amylase activity in the specimen and is Measured photometrically

Directions :

Reaction type ………………Fixed point
Reaction direction…………… ………….. Increasing
Wavelength ……………..540 nm.
Flow Cell temperature ………………..37°C
Zero setting with ……………………………………. Distilled water
Delay time ………. ……………..5 seconds
No. of readings ……………………………2
Interval ………………………………………. ………….120 seconds
Sample volume ………… ………………7 ul
Working solution volume (4R1 + 1R2) …………………1.0 ml
Linearity ……… ……………………150 mg/L

Quality Control :

To ensure adequate quality control, it is recommended that each laboratory should use a normal and an abnormal commercial reference control material. It should be realized that the use of quality control material checks both reagent and instrument functions together.

Factors which might affect the performance of this test include instrument function, temperature control, cleanliness of glasswares and accuracy of pipetting.