Buy AutoPure GPT (ALT) 220 (R1 4 X 44ml: R2 4 X 11 ml) from Accurex.

Glutamic Pyruvic Transaminase Reagent

is used for determination of alanine transaminase (ALT) in human serum and plasma on automated clinical chemistry analyzers.

AutoPure GPT (ALT) Reagent is a ready-to-use, two liquid reagent system & the assay is linear upto linear upto 600 IU/ (10.02 kati). Accurex Biomedical is the first Biochemical reagents manufacturing company in India.

About :

• AutoPure ALT (GPT) is reagent kit for direct quantitative determination of alanine transaminase (ALT) in human serum and plasma on automated clinical chemistry analyzers.
• AutoPure ALT (GPT) isa ready-to-use, two liquid reagent system.
• With AutoPure ALT (GPT), the assay is linear upto 600 IU/ (10.02 kati).

Principle :

α-ketoglutarate reacts with L-aspartate in the presence of GPT (ALT) to form oxaloacetate and L-glutamate.
The increase in oxaloacetate is determined in an indicator reaction catalyzed by malate dehydrogenase.
The conversion of NADH to NAD+ is proportional to the GPT (ALT) activity in the specimen and is determined kinetically.

Procedure :