Acid Phosphates 8

Description

About:

• Autozyme Acid Phosphate is a reagent set for determination of Acid Phosphatase activity based on Kinetic method using a-naphthyl phosphate
• Autozyme Acid Phosphate is a single reagent system using one step procedure
• Autozyme Acid Phosphate can be determined in just 8 minutes at 37 degree C
  
• Acid Phosphate can be used on any Spectrophotometer, Discrete semi automated and Automated Analyzer. Programme can be designed for any specific analyzer upon request.

Pack Size : 8 x 1 ml

Principle:

In acidic pH of the buffer system, Acid Phosphatase hydrolyses a-naphthyl phosphate to a-naphthol and phosphate. The o-naphthol is then coupled with Diazotized Fast Red TR to form a Diazo dye which has strong absorbance at 405 nm. The increase in absorbance is directly proportional to the level of acid phosphatase in serum.
The addition of L-Tartrate inhibits prostatic acid phosphatase but does not inhibit other isoenzymes.
The activity of Prostatic Acid Phosphatase is obtained by subtracting the result of Non prostatic Acid phosphatase (Determination with Tartrate) from Total Acid Phosphatase (without tartrate).

Procedure:

Reaction type…… Kinetic
Reaction direction ……..Increasing
Wavelength …..405 nm.
Flow Cell temperature ……… 37°C
Zero setting with …..Distilled water
Delay time ……………….. 300 seconds
No. of readings …………..4
Interval Blank absorbance limit Sample volume …………… 60 seconds
Reagent volume ……1.0 ml
Factor ………. 743
Linearity …. .. 75 IU/I

Quality Control:

To ensure adequate quality control it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect
ne performance of this test includes proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

The kit should be stored at 2-8 °C and is stable till the expiry date indicated on the label.
The working solutions of —- Non Prostatic Acid Phosphatase & Total Acid Phosphatases are stable for 7 days at 2-8 °C.