Alkaline Phosphatase 60

Description

About:

• Autozyme Alkaline Phosphatase is a reagent set for determination of Alkaline Phosphatase activity based on kinetic method using p-nitrophenyl phosphate (p-NPP)
• Autozyme Alkaline Phosphatase is a single reagent system using one step procedure
• Autozyme Alkaline Phosphatase has one step reconstitution. It involves dissolving substrate tablet in diluent
• Autozyme Alkaline Phosphatase is a Highly stable reagent
• Autozyme Alkaline Phosphatase activity can be determined in just 2.5 minutes

Pack Size : 12 x 5 ml

Principle:

The working solution is stablə for 21 days at 2- 8°C.
The working solution should be prepared and stored in the dark (working solution bottle) provided.This is critical because the reagent is light sensitive.
It should therefore be kept away from direct light.
Alkaline Phosphatase cleaves p-nitrophenyl phosphate (p-NPP) into
p-nitrophenol and phosphate. p-nitrophenol is a yellow color compound in alkaline medium and absorbs light at 405 nm. The rate of increase in absorbance at 405 nm. is proportional to Alkaline phosphatase activity in specimen.

Procedure:

Reaction type …………………. Kinetic
Reaction direction …………………. Up
Wavelength …………………. 405 nm
Flow cell temperature …………………. 37°C(±0.2°C)
Zero setting with ………………….Working solution
Delay time ………………….60 seconds
No. of readings …………………. 4
Interval …………………. 30 seconds
Blank absorbance limit …………………. < 0.700 Abs.
Sample volume …………………. 0.02 ml (20 µl)
Reagent volume …………………. 1.0 ml
Factor …………………. 2720
Linearity ………………….700 IUl/l

Quality Control:

To ensure adequate quality control, it is recommended that each batch should include normal and abnormal commercial reference control serum.
It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and
accuracy of pipetting.

The kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
The working solutions of Non Prostatic Acid Phosphatase & Total Acid Phosphatases are stable for 7 days at 2-8°C.