ASO (TurbiPak)

PRINCIPLE:

The reagent consists of suspension of latex particles homogenous size sensitized with anti- ASO, capable of aggregation in the presence of ASO. This aggregation process produces an increase in the size of latex particles which in turn produces an increases in absorbance of the system.

SHELF LIFER:

eagents are stable up to 2-8°C intill the expiration datestated on the bottle for unopened Bottles. Protect the reagent from light and contamination.

SAMPLE:

Fresh sera or stored at 2-8°C for no longer than 48hrsIt is necessary to freeze the sample when the assay isto be carried out after that period of time. Discard contaminated and hemolyzed sample.

WORKING REAGENT PREPATION:

Prepare working reagent by mixing 4 parts R1-ASO & 1 Part of R2- ASO. Stability of working reagent is stable for 7 days at 2-8°C.

REAGENT:

Ready to use, Two Reagents system.

CALIBRATION:

Calibration type : Spline (5 points) Prepare the dilutions of calibrators in given belowmanner for calibration.

PROCEDURE :

The samples and reagents should be brought to room temperature prior to use.

LIMITATIONS & PRECAUTIONS:

● Storage conditions as mentioned on the kit tobe adhered. ● Do not freeze or expose the reagents to highertemperature as it may affect the performance of thekit. ● Before the assay bring all the reagents to theroom temperature. ● Avoid contamination of the reagent during assayprocess. ● Use clean glassware free from dust and debris.● Reagent to sample ratio as mentioned abovemust be strictly adhered, as any changes itaffects the factor and result.