About :

• AutoPure Urea/BUN is reagent kit for direct quantitative determination of Urea/BUN in human serum and plasma on automated clinical chemistry analyzers.
• AutoPure Urea/BUN is a ready-to-use, two liquid reagent system.
• With AutoPure Urea/BUN, the assay is linear upto urea 535 mg/dl (88.8 mmol/l)/urea nitrogen 250 mg/dl (41.5 mmol/l).
  Serum/Plasma Urea: 10-45 mg/dl (1.7 -7.5 mmol/l) Urea Nitrogen (BUN):5–21 mg/dl (0.84–3.5 mmol/l)

Principles :

Urea is hydrolysed by urease to form ammonia and carbon dioxide.

Quality Control :

Each batch of AutoPure Bun/Urea 240
is assayed with at least six quality control sera – Precinom Precipath*, Bio Rad |” Biorad It* Accutrol Normal and Accutrol
Abnormal prior to release
To ensure adequate quality control, it is recommended that the laboratory should Use a normal and abnormal commercial reference control serum. It should Be realized that the use of quality control material checks both the reagent and
instrument functions together.
H the control values fall outside the specified limits, each of the below criteria should be cross checked and corrected:

-Proper instrument function wavelength setting, light sourCe and temperature
-Cleanliness of probes anc cuvettes.
-Bacterial contamination of wash water used by the instrument
-Expiry date of the reagent kt.
-Brand naTIE of Roche Diagnostics, CermaryBrand name a Biorad. USA,subiect to
availability of control serum.