About :

• AutoPure Calcium Arsenazo III is a reagent kit for direct quantitative determination of calcium in human serum and plasma on automated clinical chemistry analyzers.
• AutoPure Calcium Arsenazo III is ready-to-use, single liquid reagent system
• With AutoPure Calcium Arsenazo III, the assay is linear upto 15 mg/dl (3.75 mmol/l).
• Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the calcium results should always be assessed in conjunction with the patient’s medical history, clinical examinations and other findings.
  R1 = 250 ul Sample = 5 ul

Principles :

Arsenazo Ill reacts with calcium ions at neutral pH to form a blue coloured complex. Neutral pH Ca **+Arsenazo III – Calcium-arsenazo complex Fully automated systems automatically calculate the Calcium concentration of each sample. Results in mmol/l = Results in mg/dlx0.25 With AutoPure Calcium Arsenazo III, the assay is linear upto 15 mg/dl (3.75 mmol/l). Determine samples with higher concentrations via the rerun function. On instruments without rerun function, manually dilute samples with higher concentrations using 0.9% NaCl or distilled/deionized water (e.g. 1 + 2). Multiply the result by the appropriate dilution factor(e.g. 3).

Procedure :