About :

• AutoPure chloride is a reagent kit for direct quantitative determination of Chloride in human serum and plasma on automated clinical chemistry analyzers.
• AutoPure Chloride is a ready-to-use, single liquid reagent system.
• With AutoPure Chloride, the assay is linear upto 130 mmol/l (458.9 mg/dl).

Principle :

Fully automated systems automatically calculate the Chloride concentration of each sample. Results in mg/dl = Results in mmol/l x 3.53 Chloride ions react with mercuric thiocyanate to form undissociated mercuric chloride and free thiocyanate ions.

Procedure :

AutoPure Chloride can be used on various automated analyzers. The procedure described below is for Sphera auto-analyzer.
493 Sec. 37°C 6 Measurement at 505 nm Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range. For diagnostic purposes, the Chloride results should always be assessed in conjunction with the patient’s medical history, clinical examinations and other findings.

Quality Control :

Each batch of AutoPure Chloride 80 is assayed with at least six quality control sera-Precinorm**, Precipath**, Bio Rad I***, Bio Rad II***, Accutrol Normal and Accutrol Abnormal prior to release-, To ensure adequate quality control, it is recommended that the laboratory should use a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both the reagent and instrument functions together. If the control values fall outside the specified limits, each of the below criteria should be cross-checked and corrected:

• Proper instrument function – wavelength setting, light source and temperature control.
• Cleanliness of probes and cuvettes.
• Bacterial contamination of wash water used by the instrument.
A comparison of the calcium determination using AutoPure Calcium Arsenazo III and AutoZyme Calcium Arsenazo III reagent gave the following correlation (mg/dl): Linear Regression y=0.949x+0.574 r=0.941 Sy.x=0.33