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AutoPure GOT (AST) 220 (R1 4 X 44ml: R2 4 X 11 ml) from Accurex.

Glutamic Oxaloacetic Transaminase Reagent

is used for direct quantitative determination of aspartate transaminase (AST) in human serum and plasma on automated clinical chemistry analyzers.

AutoPure AST (GOT) Reagent is a ready-to-use, two liquid reagent system & the assay is linear upto 800 IU/I (13.36 μkat/I). Accurex Biomedical is the first Biochemical reagents manufacturing company in India.

About :

• AutoPure AST (GOT) is a reagent kit for direct quantitative determination of aspartate transaminase (AST) in human serum and plasma on automated clinical chemistry analyzers.
• AutoPure AST (GOT) is a ready-to-use, two liquid reagent system.
• With AutoPure AST (GOT), the assay is linear upto 800 IU/I (13.36 μkat/I).

Principle :

α-ketoglutarate reacts with L-aspartate in the presence of AST (GOT) to form oxaloacetate and L-glutamate.
The increase in oxaloacetate is determined in an indicator reaction catalyzed by malate dehydrogenase.
The conversion of NADH to NAD+ is proportional to the AST (GOT) activity in the specimen and is determined kinetically.

Procedure :