AutoPure AST (GOT) is a ready-to-use, two liquid reagent system.
Shelf – Life Stable till the expiry date indicated on the label, when stored at 20 – 80C.
On – Board Reagent Stability
R1 : 30 days at 20 – 80C after opening. Protect the reagent from light and contamination.
R2 : 30 days at 20 – 80C after opening. Protect the reagent from light and contamination.
Do not freeze the reagent.
Buy
AutoPure GOT (AST) 220 (R1 4 X 44ml: R2 4 X 11 ml) from Accurex.
is used for direct quantitative determination of aspartate transaminase (AST) in human serum and plasma on automated clinical chemistry analyzers.
AutoPure AST (GOT) Reagent is a ready-to-use, two liquid reagent system & the assay is linear upto 800 IU/I (13.36 μkat/I). Accurex Biomedical is the first Biochemical reagents manufacturing company in India.
About :
Principle :
α-ketoglutarate reacts with L-aspartate in the presence of AST (GOT) to form oxaloacetate and L-glutamate.
The increase in oxaloacetate is determined in an indicator reaction catalyzed by malate dehydrogenase.
The conversion of NADH to NAD+ is proportional to the AST (GOT) activity in the specimen and is determined kinetically.
Procedure :
AutoPure AST (GOT) can be used on various automated analyzers.
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