Buy AutoPure Phosphorous 80 (Pack Size: 4 X 20ml) from Accurex.

AutoPure Phosphorous 80 Reagent

is used for direct quantitative determination of phosphorus in human serum and plasma on automated clinical chemistry analyzers.

AutoPure Phosphorus 80 Reagent is a ready-to-use, single liquid reagent system & With Phosphorous 80 the assay is linear upto 20 mg/dl (6.46 mmol/l). Inorganic Phosphorus reacts with Ammonium molybdate in strong acidic medium -> forms a Phosphor Inorganic Molybdate complex. Absorbance of this complex is directly proportional to phosphorus concentration.

About :

• AutoPure Phosphorus is a reagent kit for direct quantitative determination of phosphorus in human serum and plasma on automated clinical chemistry analyzers.
• AutoPure Phosphorus is a ready-to-use, single liquid reagent system.
• With AutoPure Phosphorus, the assay is linear upto 20 mg/dl (6.46 mmol/l). Fully automated systems automatically calculate the Phosphorus concentration of each sample.

Principle :

Inorganic Phosphorus reacts with Ammonium molybdate in strong acidic medium to form a Phospho-Inorganic Molybdate complex. The absorbance of this complex is directly proportional to the phosphorus concentration.

Procedure :

● Discard the working reagent if the absorbance of the same is more than 0.300 against distilled water at 340 nm
● If the phosphorus value exceeds the linearity limit then dilute the specimen suitably with normal saline and repeat the assay. In such case, the assay value should be multiplied by the dilution factor to obtain the correct phosphorus value of the specimen.
● Strong lipemic and hemolytic sera should not be used.
● Contaminated glassware is the greatest source of error. Disposable plastic tubes and clean glassware are recommended for the test.

● The reagent contains sulphuric acid. Avoid contact with skin and mucous membrane. If you come in contact with the reagent wash thoroughly with water.

Quality Control :

To ensure adequate quality control, it is recommended that each assay should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors that might affect the performance of this test include proper instrument function, cleanliness of glassware, and accuracy of pipetting.