Calcium Arsenazo III 150

Description

About:

• Single ready-to-use liquid reagent
• Kit storage at R.T.
• End point assay: 2 minutes
• Autozyme Calcium Arsenazo III is a reagent set for determination of total calcium concentration in serum based on the colorimetric method using Arsenazo III
• Autozyme Calcium Arsenazo III is a ready to use reagent
• Autozyme Calcium Arsenazo III estimates calcium in just 2 minutes at room temperature
• Autozyme Calcium Arsenazo III is linear up to 15mg%
• The influence of lipids, haemolysis and bilirubin is negligible
  Pack Size : 3 x 50 ml

Principle:

Calcium with Arsenazo III at neutral pH yields a blue coloured complex. The intensity of the color formed is directly proportional to calcium concentration.

Procedure:

Reaction type …………End-Point
Reaction time …………… 2 mins. at R.T.(25-30°C)
Wavelength ……………. …………. 650 nm.(620 – 650 nm.)
Zero setting with ………………. Reagent Blank
Blank absorbance limit …………………………. <0.800 Abs.
Sample volume ………… 0.02 ml (20 ur)
Reagent volume ………………… 1.0 ml
Standard concentration ………… 10 mg%
Linearity …………… 15 mg/dl

Quality Control:

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

The kit should be stored at R.T. (25-30°C) and is stable till the expiry date indicated on the label.