Chloride 100 – Autozyme

Description

About:

• AutoZyme Chloride is a reagent set for determination of chloride in serum/plasma based on Mercuric thiocyanate method.
• AutoZyme Chloride is a single reagent system, ready-to-use.
• AutoZyme Chloride estimates chloride in just 1 minute.
• AutoZyme Chloride is linear upto 130 mmol/l.
• AutoZyme Chloride can be used on any Spectrophotometer, Discrete semiautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
• The shelf-life of AutoZyme Chloride is 15 months.

Principle:

Chloride reacts with mercuric thiocyanate to give mercuric chloride. The thiocyanate which is released reacts with ferric ions present in the reagent to form ferric thiocyanate which is directly proportional to chloride present in serum/plasma & can be measured at 510 nm.(500 – 520 nm.).

Procedure:

Reaction type ………………………………………………….. End Point
Reaction time……………………………… 1 min. at R.T. (25-30°C)
Wavelength ………………………………….. 510 nm.(500-520 nm.)
Zero setting with ……………………………………. Reagent Blank
Blank absorbance limit ………………………………. < 0.200 Abs.
Sample volume …………………………………………0.01 ml (10 μl)
Reagent volume ……………………………………………………. 1.0 ml
Standard concentration ……………………………….. 100 mmol/l
Linearity ………………………………………………………… 130 mmol/l

Incubation:
Incubate the assay mixture for 1 minute at room temperature
(25-30°C). After completion of incubation period measure the
absorbance of assay mixture against blank at 510 nm. The final
colour is stable for one hour if not exposed to direct light.

Quality Control:

To ensure adequate quality control, it is recommended that each assay should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both, instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, cleanliness of glassware and accuracy of pipetting.

The specimen to reagent ratio can be altered proportionally without affecting the results.