CK MB 30 – Infinite

Description

About:

• Infinite Liquid CK – MB is a reagent set for determination of CK – MB activity in serum and plasma based on immunoinhibit ion methods.
• Infinite Liquid CK – MB is a ready-to-use, two liquid reagent system.
• Infinite Liquid CK-MB estimates CK – MB activity in just 612 minutes.
• Infinite Liquid CK – MB is linear up to 2000 IU/.
• Infinite Liquid CK – MB can be used on any Spectrophotometer, Discrete semi automated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
• Infinite Liquid CK – MB is stable till expiry at 2 -8° C.

Principle:

The serum sample is incubated with CK – MB reagent containing an antibody specific to CK-M subunit which completely inhibits the CK-M monomer. The activity of CK-B which is not inhibited by the antibody, is then measured by the following reaction sequence:
СК-В Creatine phosphate + ADP = creatine + ATP

Procedure:

Reaction type …………… UV – Kinetic
Reaction direction …… .. Increasing
Wavelength …….. ……. 340 nm.
Flow Cell temperature. …
Zero setting with …. ………. Distilled water
Delay time ………………………………………………… 300 seconds
No. of readings … …………………………4
Interval ……………. …………….. 30 seconds
Sample volume ………………0.05 ml (50 ul)
Working solution volume (4R, : 1R) ……… 1.0 ml (1000 ul)
Factor ….. … 6752
Linearity 2000 IU/

Quality Control:

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of the test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

The reagent kit should be stored at 2 -8° C and is stable till the expiry date indicated on the label. R1 & R2 reagents are stable till expiry at 2 -8° C. The working solution (4 R1 + 1 R2) is stable for 10 days at 2 – 8° C.