CK NAC 30 – Infinite

Description

About:

• Infinite Liquid CK – NAC is a reagent set for determination of creatine kinase activity in serum and plasma based on UV – Kinetic method.
• Infinite Liquid CK – NAC is a ready-to-use, two liquid reagent system.
• Infinite Liquid CK – NAC estimates creatine kinase activity in just 472 minutes.
• Infinite Liquid CK – NAC is linear upto 2000 IU/.
• Infinite Liquid CK – NAC can be used on any Spectrophotometer, Discrete semi automated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
• Infinite Liquid CK – NAC is stable till expiry at 2 – 8°C.

Principle:

Manual assay procedure Prewarm at 37°C the required amount of working solution be fore use. Perform the assay as given below :
Creatine kinase catalyzes the conversion of creatine phosphate and ADP to creatine and ATP. ATP phosphorylates glucose to glucose-6-phosphate in the presence of hexokinase. Glucose-6-phosphate is oxidized to 6-phosphogluconate, reducing NADP to NADPH in presence of G-6-PDH. The rate of increase in NADPH absorbance at 340 nm. is directly proportional to the activity of creatine kinase in serum/plasma.

Procedure:

Reaction type ………… UV – Kinetic
Reaction direction ………….. …………… Increasing
Wavelength ……… ………………… 340 nm.
Flow Cell temperature ………. ….37°C
Zero setting with …………………………………… Distilled water
Delay time ……….. ……. …………………….. 180 seconds
No. of readings ……………. …………………………4
Interval ……….. … …. …….. 30 seconds
Sample volume …………… ……………… …………0.04 ml (40 ul)
Working solution volume (4R, : 1R,) ……. 1.0 ml (1000 ul)
Factor ……………………….. 4127 0
Linearity. ………………… 2000 IU/

Quality Control:

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting

The reagent kit should be stored at 2 -8° C and is stable till the expiry date indicated on the label. R1 & R2 reagents are stable till expiry at 2 -8° C. The working solution (4 R1 + 1 R2) is stable for 10 days at 2 – 8° C.