Diluent Glucose Eco-Pak 500

Description

About:

• Eco- Pak Glucose is a reagent set for determination of True Glucose, based on enzymatic methods using Glucose oxidase and Peroxidase.
• Eco- Pak Glucose has one step reconstitution. It involves the mixing of Enzyme and Diluent.
• Eco- Pak Glucose is linear upto 450 mg%.
• Eco- Pak Glucose is a High Stability Reagent.
• Eco- Pak Glucose can be used on any Spectrophotometer, Discrete semi automated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
• Eco- Pak Glucose can be determined in just 15 minutes at 37°C or 30 minutes at A.T. (25-30°C).
• Sodium Fluoride (as an anticoagulant upto 10 mg/ml blood) does not affect glucose assay.
• The influence of Ascorbate, Bilirubin, Antidiabetic drugs and Hemoglobin is negligible.
• The reagent can also determine glucose using whole blood (0.02 ml) collected from finger-prick. The procedure is available upon request.

Priniple:

Glucose oxidase (GOD) converts glucose to gluconic acid. Hydrogen peroxide formed in this reaction, in presence of peroxidase (POD), oxidatively couples with 4-aminoantipyrine and phenol to produce red quinoneimine dye. This dye has absorbance maximum at 505 nm. (500 – 550 nm.). The intensity of the color complex is directly proportional to the concentration of glucose in the specimen.

Procedure:

Reaction type ………… End-Point
Reaction time …. 15 mins. at 37°C/30 mins. at R.T. (25 -30°C)
Wavelength ……………….. 505 nm.(500-550 nm.)
Zero setting with ……….. ……….. Working Solution
Sample volume …………………………….0.01 ml (10 ul)
Reagent volume ………..1.0 ml

Standard concentration …… ……… 100 mg%
Linearity ……….. …………… 450 mg/dl

Quality Control:

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use to quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

The working solution is stable for 60 days when stored at 2 -8°C (DO NOT FREEZE). The working solution should be stored in the dark bottle (working solution container) provided. This is critical because the reagent is light sensitive (auto oxidation of chromogen system by light and air), it should therefore be kept away from direct light.