G6PDH Qualitative Dye 12

Description

About:

• AutoZyme NEW G6PDH (Qualitative-Dye) is a reagent set for qualitative determination (screening) of G6PDH deficiency in erythrocytes by decolourisation method. Estimate Hemoglobin content (gm/dl) of whole blood. If the hemoglobin content is significantly less than 15 gm/dl, adjust the hemoglobin content by proportionately increasing the aliquot of whole blood during preparation of red cell hemolysate.
• AutoZyme NEW G6PDH (Qualitative-Dye) is a reagent set with simple
  procedure and easy technique.
• AutoZyme NEW G6PDH (Qualitative-Dye) is available in convenient

pack-size of 12 tests

Principle:

Glucose-6-Phosphate Dehydrogenase (G6PDH) present in hemolysate acts on substrates, Glucose-6-Phosphate and NAD, giving NADPH, which in presence of PMS (Phenazine methosulfate) decolorises blue coloured indophenol dye (DCPIP) leaving behind color only due to hemolysate.
The rate of reaction is proportional to G6PDH present, hence time required for decolorization is inversely proportional to G6PDH activity in the hemolysate

Procedure:

Estimate Hemoglobin content (gm/di) of whole blood. f the
hemoglobin content is significantly less than 15 gm/dl, adjust
the hemoglobin content by proportionally increasing the
aliquot of whole blood during preparation of red cell hemolysate.

Quality Control:

To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control the serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature
control, cleanliness of glassware and accuracy of pipetting.

Freshly prepared working reagent should be used.
The reagent kit should be stored at 2- 8°C & is stable till expiry date
indicated on the label. DO NOT FREEZE THE REAGENT.