Glucose STAT 2000

Description

About:

• Long liquid stability : 12 months
• Both End-Point and Kinetic method possible
• End-Point : 7 minutes
• Linearity : 500 mg%
• Kinetic : 1 minute
• Linearity : 700 mg%
• Rock-steady reagent blank
• Results via factor possible
• 2 standards provided : 100 mg% & 300 mg%
• Autozyme Stat Glucose is a reagent set for determination of true glucose based on enzymatic method using Glucose oxidase and peroxidase
• Autozyme Stat Glucose estimates glucose in 1 minute using an initial rate method and in 7 minutes by end-point method
• Autozyme Stat Glucose is linear up to 700mg% glucose for kinetic assay procedure and 500mg% using end point procedure
• Autozyme Stat Glucose has one step reconstitution. It involves mixing Enzyme and Diluent
• Pack Size : 10 x 200 ml

Principle:

Glucose oxidase (GOD) converts Glucose to gluconic acid. Hydrogen peroxide formed in this reaction, in presence of Peroxidase (POD), oxidatively couples with 4-aminoantipyrine / phenol to produce red quinoneimine dye. This dye has absorbance maximum at 505 nm. (500-550 nm.). The intensity of the color complex is directly proportional to the Glucose in the specimen.

Procedure:

Reaction time ………7 mins. at 37 C/15 mins. at R.T. (25-30 C)
Wavelength …………………………………….505 nm. (500-550 nm.)
Zero setting with…………………………………………Reagent Blank
Sample volume…………………………………………….0.01 ml (10μl)
Reagent volume………………………………………………………1.0 ml
Standard concentration ………………………………………100 mg%
Linearity ……………………………………………………………500 mg/d

Quality Control:

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of the test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

Stability of the reconstituted working reagent is printed on the bottle label. (DO NOT FREEZE). The working solution should be stored in the dark bottle (diluent bottle or the working solution bottle) provided. This is critical because the reagent is light sensitive (auto oxidation of chromogen system by light and air), it should therefore be kept away from direct light.