GPT 200

Description

About:

• Autozyme GPT is a reagent set for determination of GPT based on UV-Kinetic method recommended by IFCC
• GPT activity can be determined in 2.5 minutes
• Autozyme GPT is linear up to 400 IU/I and has high stability
• The shelf life of Autozyme GPT is 18 months

Principle:

The conversion of NADH to NAD in GPT reaction, is proportional to the concentration of GPT in serum and is measured at 340 nm, as Tate of decrease in absorbance.

Procedure:

Reaction type ……………………… UV – Kinetic
Reaction direction …………………………… …. ……… Down
Wavelength ……………. …………….. 340 nm.
Flow Cell temperature ……….. ……………. 37°C O
Zero setting with ………………… Distilled water
Delay time. ………….. 60 seconds
No. of readings ……………………………………….4 0
Interval …… ………… 30 seconds
Blank absorbance limit ……………….. 20.900 Abs.
Sample volume ……………………………… .. 0.1 ml (100 ul)
Reagent volume ………………….. 1.0 ml
Factor …………………………………. 1749
Linearity ……………………….. 400 IUA

Quality Control:

To ensure adequate quality control. it is recommended that each batch should include a normal and abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature Control, cleanliness of glassware and accuracy of pipetting.

The reagent kit should be stored at 2-8°C and is stable till the expiry date indicated on the label.
The GPT working solution is stable tor 60 days at 2- 8°C, Do not freeze the working solution.