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HbA1c

HbA1c

The term HbA1c refers to glycated haemoglobin and its levels are reflective of blood glucose levels over the past six to eight weeks. This method utilizes the interaction of antigen and antibody to determine HbA1c concentration in the EDTA whole blood.

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Description

The term HbA1c refers to glycated haemoglobin and its levels are reflective of blood glucose levels over the past six to eight weeks. This method utilizes the interaction of antigen and antibody to determine HbA1c concentration in the EDTA whole blood.

Salient Features:

  • Latex Enhanced Immunoturbidimetry method provides high sensitivity and specificity
  • No interference of Hemoglobin variants HbA2, HbC and HbS
  • High Linearity upto 18%
  • Two reagents for ensuring stability with no reagent preparation
  • High correlation with HPLC method
  • Valid for semi-automated as well as fully automated analyzer

Features:

 

  • Latex Enhanced Immunoturbidimetry method – uses highly specific antibody to A1c which provides high sensitivity and specificity.
  • Two reagents for ensuring stability with no reagent preparation.
  • Certified by the NGSP%, reference to IFCC.
  • No interference of Hemoglobin variants HbA2 ,HbC and HbS.
  • Assay procedure valid for semi automated as well as fully automated analyzer.
  • High Linearity upto 18%.
  • High correlation with HPLC method

Principle:

This method utilizes the interaction of antigen and antibody to determine HbA1c concentration in the EDTA whole blood. HbA1c in test sample is absorbed onto the surface of the latex particles, which reacts with Anti-HbA1c ( Antigen antibody reaction ) and gives agglutination. The amount of agglutination is measured as absorbance. The HbA1c value is obtained from calibration curve.

Assay Procedure:

 

  • Take the hemolyzed sample.

  • Add Reagent R1 to the sample.

  • Wait for 5 minutes.

  • Add Reagent R2 to the mixture.

  • Wait for another 5 minutes.

  • Record the results.

Assay Interference:

No interference was observed for

  • This method utilizes the interaction of antigen and antibody to determine HbA1c concentration in the EDTA whole blood. HbA1c in test sample is absorbed onto the surface of the latex particles, which reacts with Anti-HbA1c ( Antigen antibody reaction ) and gives agglutination. The amount of agglutination is measured as absorbance. The HbA1c value is obtained from calibration curve.
  • Ascorbic Acid : up to 50mg/dL .
  • Bilirubin : up to 50mg/dL.
  • Triglyceride : up to 2000mg/dL.
  • Carbamylated Hb : up to 7.5mmol/L.
  • Acetylated Hb : up to 5.0mmol/L

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