LDL – Cholesterol Direct 40 – Infinite

Description

About:

• Infinite LDL-C is a reagent set for direct quantitative determination of LDL-Cholesterol in human serum or plasma based on Direct Homogenous method.
• Direct measurement by infinite LDL-C overcomes the limitation of Fried Ewald formula thereby allowing accurate determination of LDL-C.
• Infinite LDL-C is a ready-to-use, two liquid reagent system stable till expiry.
• Infinite LDL-C is linear upto 450 mg/di.
• There is no significant interference in hyper-lipemic and hyperimmunoglobulin samples.

Principle:

In the first step, HDL, VLOL & Chylomicrons are eliminated under specific conditions.
In the second step, special reagents break up LDL and make LDL Cholesterol available for measurement.

Procedure:

Reaction type ……… End-Point (2 Step)
Reaction time ………………5 + 5 mins
Wavelength……………. 546 nm.
Flow Cell temperature……………….. 37°C
Zero setting with ……………………… Distilled water
Sample volume ……………. Q.01 ml (10 µt)
Reagent volume 0.750 ml + 0.250 ml
Linearity ………….. .. 450 mg/dl

Quality Control:

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference SPECIMEN COLLECTION & PRESERVATION

The reagent kit should be stored at 2 – 8°C and is stable till the expiry date indicated on the label. Reconstituted calibrator is stable for 3 days at 2′ – 8°C. Reconstitution stability of the calibrator may be extended upto 30 days by aliquoting and freezing the reconstituted calibrator preparation at less than -20°C.