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(Pack Size: 5 X 50 ml) from Accurex. Liquid Triglycerides reagent kit is used for determination of triglycerides based on enzymatic methods using Lipoprotein lipase, Glycerol kinase, Glycerol phosphate oxidase and Peroxidase.
Triglyceride Reagent is a ready to use reagent & can be determined in just 10 minutes at 37°C or 20 minutes at Room Temperature (25°C – 30°C). The Triglyceride kit should be stored at 2- 8°C and is stable till the expiry date indicated on the label. Triglycerides normal range is Less than 150 milligrams per deciliter (mg/dL) or less than 1.7 millimoles per liter (mmol/L).
Method – GPO-POD
Pack Size : 5 x 25 ml
Glycerol released from hydrolysis of triglycerides by lipoprotein lipase is Converted by glycerol kinase into glycerol- 3- phosphate which is oxidized by glycerol phosphate oxidase to dihydroxyacetone phosphate and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidizes phenolic chromogen to a red coloured compound.
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
The kit should be stored at 2- 8°C and is stable till the expiry date indicatedon the label.The reagent and standard are ready-to-use and are stable till expiry, whenstored at 2-8°C. DO NOT FREEZE THE REAGENT.
The reagent should be stored only in the amber bottle provided to protect it from direct light. Before use swirl in the reagent gently. DO NOT SHAKE VIGOROUSLY.
Over time, the reagent may develop a light pink color. This is expected and does not affect the reagent performance. Discard the reagent if the absorbance of the same exceeds 0.300O.D. against distilled water at 510 nm. Contamination of the reagent should be strictly avoided. Should the reagents develop turbidity and discard the reagent.
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