BUN 500

Description

About:

• Infinite Liquid BUN (BUN 500– Liquid) is a reagent set for determination of blood urea nitrogen (BUN) concentration in serum or plasma based on Enzymatic UV- Kinetic initial rate method.
• Infinite Liquid BUN is a ready-to-use, two liquid reagent system.
• Infinite Liquid BUN estimates urea nitrogen concentration in just 1 minute.
• Infinite Liquid BUN is linear upto 250 mg/dl.
• Infinite Liquid BUN can be used on any Spectrophotometer, Discrete Semiautomated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
• Infinite Liquid BUN is stable till expiry at 2 -8°C.
  Method – GLDH-Kinetic
  Pack Size : R1: 4 x 100 ml R2:2x50ml

Principle:

Urea is hydrolyzed to ammonia and carbon dioxide by urease. Ammonia produced reacts with @-ketoglutarate to form glutamate in presence of glutamate dehydrogenase. NADH is oxidized to NAD* in this reaction, which Is measured as a decrease in absorbance at 340 nm. The rate of decrease in absorbance at 340 nm. is directly proportional to BUN concentration in the specimen. The BUN concentration in the specimen is determined by comparing the sample reaction rafe to that obtained with a BUN standard

Procedure:

Reaction type ………………UV – Kinetic
Reaction direction ………Decreasing
Wavelength ………340 nm.
Flow Cell temperature ………25°C/30°C
Zero setting with ………Distilled
water Delay time ………30 seconds
No. of readings ………2
Interval ………30 seconds
Blank absorbance limit ………21.000 Abs.
Sample volume ………0.01 ml (10 ul)
Working solution volume (4 R, : 1 R.) ………1.0 ml (1000 ul)
Factor ………20 = (A Abs. of Standard)
Linearity ………250 mg/dl

Quality Control:

To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect include proper instrument function, temperature, the performance of this test control cleanliness of glassware and accuracy of pipetting.

The reagent kit should be stored at 2- 8 C and is stable till the expiry date indicated on the label. The working solution is stable for 45 days at 2- 8°C.