HDL Cholesterol Direct 80

Description

About:

• Infinite HDL-C is a reagent set for direct quantitative determination of HDL-Cholesterol in human serum or plasma based on Direct Homogenous method.
• Direct measurement by Infinite HDL-C overcomes the limitation of precipitation method thereby allowing accurate determination of HDL-C.
• Infinite HDL-C is a ready-to-use, two liquid reagent system stable till expiry.
• Infinite HDL-C is linear upto 185 mg/dl.
• There is no significant interference in hyper-lipemic and hyper immunoglobulin samples
• Results obtained with Infinite HDL-C show excellent correlation with ultracentrifugation methods.

Principle:

In the first step, LDL, VLDL & Chylomicrons are eliminated under specific conditions.
Mix and incubate for 5 minutes at 37°C. Measure the absorbance A1 at 578 nm.In the second step, special reagents break up HDL and make HDL Cholesterol available for measurement.

Procedure:

Reaction type ………………. End Point (2 Step)
Reaction time ……………….. 5 + 5 mins.
Wavelength ……………………………….. 578 nm.
Flowcell temperature ……………………….. 37°C
Zero setting with ………………………….. Distilled water

Sample volume ……………… 0.01 ml (10 pl)
Reagent volume ……….. ……. 0.750 ml + 0.250 ml
Linearity …. 185 mg/dl

Quality Control:

To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.

The reagent kit should be stored at 2 – 8°C and is stable till the expiry date indicated on the label. Reconstituted calibrator is stable for 3 days at 2° -8°C. Reconstitution stability of the calibrator may be extended upto 30 days by aliquoting and freezing the reconstituted calibrator preparation at less than -20°C.
Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range.