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from Accurex (Pack Size : 18 x 25 ml). Uric Acid reagent is used for determination of Uric Acid based on enzymatic method using Uricase and Peroxidase.
Autozyme Uric Acid is a single reagent system & can be determined in 8 minutes. Certain foods filled with dietary fibers are extremely beneficial in the uric acid treatment & in eliminating Uric Acid in body. Gout is a form of inflammatory arthritis which is very painful. Uric acid Reagent is stable for 3 days at room temperature (below 25°C) and for 6 months when stored at -10°C
Pack Size : 18 x 25 ml
Uricase converts uric acid into allantoin and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidatively couples with phenolic chromogens to form a red coloured compound, which has maximum absorbance at 510 nm. (500-530 nm.). The concentration of the red coloured compound is proportional to the amount of uric acid in the specimen.
Reaction type …End Point
Reaction time….. …….. 10 mins. at R.T. (25 – 30°C)
Wavelength ………………. 510 nm. (500-530 nm.)
Zero setting with …………….. Reagent Blank
Blank absorbance limit ………….. <0.300 Abs.
Sample volume ……. 0.025 ml (25 ul) 0
Reagent volume …………… 1.0 ml .
Standard concentration ………… 6 mg%
Linearity………. 25 mg/dL
Quality Control:
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control sérum.. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
In neatly separated serum/plasma, uric acid is stable for 3 days at room temperature (below 25°C) and for 6 months when stored at -10°C.
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